Web Access Research Portal

Researcher: Smith, SM (Professor Steven Smith)

Fields of Research

Plant physiology
Plant cell and molecular biology
Plant developmental and reproductive biology
Plant biochemistry
Proteomics and intermolecular interactions (excl. medical proteomics)
Other biological sciences
Crop and pasture improvement (incl. selection and breeding)
Crop and pasture biochemistry and physiology
Separation science
Plant pathology
Cell metabolism

Research Objectives

Expanding knowledge in the biological sciences
Grains and seeds
Expanding knowledge in the chemical sciences
Other plant production and plant primary products
Protected vegetable crops
Grain legumes
Environmentally sustainable plant production
Clinical health
Expanding knowledge in the agricultural, food and veterinary sciences
Rehabilitation or conservation of terrestrial environments

Career Best Publications

F-box protein MAX2 has dual roles in karrikin and strigolactone signaling in Arabidopsis thaliana; Proceedings of the National Academy of Sciences of The United States of America.
Specialisation within the DWARF14 protein family confers distinct responses to karrikins and strigolactones in Arabidopsis; Development
Witchcraft and destruction; Nature

Research Publications

α-amylase is not required for breakdown of transitory starch in Arabidopsis leaves; Journal of Biological Chemistry
β-AMYLASE4, a noncatalytic protein required for starch breakdown, acts upstream of three active β-amylases in Arabidopsis chloroplasts; Plant Cell
ζ-Carotene Isomerase Suppresses Tillering in Rice through the Coordinated Biosynthesis of Strigolactone and Abscisic Acid; Molecular Plant
LATERAL BRANCHING OXIDOREDUCTASE acts in the final stages of strigolactone biosynthesis in Arabidopsis; National Academy of Sciences of The United States of America. Proceedings
A Selaginella moellendorffii ortholog of KARRIKIN INSENSITIVE2 functions in Arabidopsis development but cannot mediate responses to karrikins or strigolactones; Plant Cell
A cDNA encoding a putative SPF1-type DNA-binding protein from cucumber; Gene
A central role for the peroxisomal membrane in glyoxylate cycle function; Biochimica et Biophysica Acta: Molecular Cell Research
A Core Regulatory Pathway Controlling Rice Tiller Angle Mediated by the LAZY1-Dependent Asymmetric Distribution of Auxin; The Plant Cell
A critical role for disproportionating enzyme in starch breakdown is revealed by a knock-out mutation in Arabidopsis; The Plant Journal
A cytosolic glucosyltransferase is required for conversion of starch to sucrose in Arabidopsis leaves at night; The Plant Journal
A nuclear-localized protein, KOLD SENSITIV-1, affects the expression of cold-responsive genes during prolonged chilling in Arabidopsis; Journal of Plant Physiology
A number of different nuclear genes for the small subunit of RuBPCase are transcribed in petunia; Nucleic Acids Research
Acclimation-induced changes in cell membrane composition and influence on cryotolerance of in vitro shoots of native plant species; Plant Cell, Tissue and Organ Culture
An allelic series at the KARRIKIN INSENSITIVE 2 locus of Arabidopsis thaliana decouples ligand hydrolysis and receptor degradation from downstream signalling; The Plant Journal
An extra-plastidial α-glucan, water dikinase from Arabidopsis phosphorylates amylopectin in vitro and is not necessary for transient starch degradation; Journal of Experimental Botany
Arabidopsis AtcwINV3 and 6 are not invertases but are fructan exohydrolases (FEHs) with different substrate specificities; Plant, Cell and Environment
Arabidopsis has a cytosolic fumarase required for the massive allocation of photosynthate into fumaric acid and for rapid plant growth on high nitrogen; The Plant Journal
Arabidopsis peroxisomal citrate synthase is required for fatty acid respiration and seed germination; Plant Cell
Arabidopsis peroxisomal malate dehydrogenase functions in β-oxidation but not in the glyoxylate cycle; The Plant Journal
Assaying germination and seedling responses of Arabidopsis to karrikins; Plant Hormones: Methods and Protocols
Band-flip and kink as novel structural motifs in α-(1→4)-D-glucose oligosaccharides. Crystal structures of cyclodeca- and cyclotetradecaamylose; Carbohydrate Research
Biogenic production of palladium nanocrystals using microalgae and their immobilization on chitosan nanofibers for catalytic applications; RSC Advances
Breeding high-yield superior quality hybrid super rice by rational design; National Science Review
Burning vegetation produces cyanohydrins that liberate cyanide and stimulate seed germination; Nature Communications
Carlactone-independent seedling morphogenesis in Arabidopsis; The Plant Journal
Catalytically-inactive β-amylase BAM4 required for starch breakdown in Arabidopsis leaves is a starch-binding-protein; Archives of Biochemistry and Biophysics
Chapter 2: Application of various immobilization techniques for algal bioprocesses; Biomass and Biofuels from Microalgae: Advances in Engineering and Biology
Characterisation of three cDNA clones encoding different mRNAs for the precursor to the small subunit of wheat ribolosebisphosphate carboxylase; Nucleic Acids Research
Characterization of chilli pepper hairy roots expressing the parsley PAL2 cDNA; Plant Biotechnology
Chewing the fat: β-oxidation in signalling and development; Trends in Plant Science
Chromosome and gene structure in plants: a picture deduced from analysis of molecular clones of plant DNA; Genome Organization and Expression in Plants
Conservation of two lineages of peroxisomal (Type I) 3-ketoacyl-CoA thiolases in land plants, specialization of the genes in Brassicaceae, and characterization of their expression in Arabidopsis thaliana; Journal of Experimental Botany
Critical roles of soluble starch synthase SSIIIa and granule-bound starch synthase Waxy in synthesizing resistant starch in rice; Proceedings of the National Academy of Sciences of The United States of America
Cyclic glucans produced by the intramolecular transglycosylation activity of potato D-enzyme on amylopectin; Biochemical and Biophysical Research Communications
Cyclization reaction catalyzed by glycogen debranching enzyme (EC and its potential for cycloamylose production; Applied and Environmental Microbiology
Destabilization of strigolactone receptor DWARF14 by binding of ligand and E3-ligase signaling effector DWARF3; Cell Research
Developmental regulation of expression of the malate synthase gene in transgenic plants; Plant Molecular Biology
Disproportionating enzyme (4-a-Glucanotransferase; EC of potato; Journal of Biological Chemistry
Distinct cis-acting sequences are required for the germination and sugar responses of the cucumber isocitrate lyase gene; Gene
Distinct cis-acting elements direct the germination and sugar responses of the cucumber malate synthase gene; Molecular and General Genetics
Diurnal and light-regulated expression of AtSTP1 in guard cells of Arabidopsis; Plant Physiology
Diurnal changes in the transcriptome encoding enzymes of starch metabolism provide evidence for both transcriptional and posttranscriptional regulation of starch metabolism in Arabidopsis leaves; Plant Physiology
Divergent receptor proteins confer responses to different karrikins in two ephemeral weeds; Nature Communications
Does the glyoxylate cycle have an anaplerotic function in plants?; Trends in Plant Science
Editorial overview: physiology and metabolism: plant metabolism: globules to global, modules to models; Current Opinion in Plant Biology
Enhanced accumulation of microalgal pigments using metal nanoparticle solutions as light filtering devices; Green Chemistry
Entrapment of Chlorella vulgaris cells within graphene oxide layers; RSC Advances
Establishment of hairy root cultures of chilli pepper (Capsicum frutescens); Plant Tissue Culture Letters
Evidence for distinct mechanisms of starch granule breakdown in plants; Journal of Biological Chemistry
Evolution of strigolactone perception by seeds of parasitic plants: reinventing the wheel; Molecular Plant
Exploring the molecular mechanism of karrikins and strigolactones; Bioorganic and Medicinal Chemistry Letters
Expression of a single gene encoding microbody NAD-malate dehydrogenase during glyoxysome and peroxisome development in cucumber; Plant Molecular Biology
Expression of glyoxylate cycle genes in cucumber roots responds to sugar supply and can be activated by shading or defoliation of the shoot; Plant Molecular Biology
F-box protein MAX2 has dual roles in karrikin and strigolactone signaling in Arabidopsis thaliana; Proceedings of the National Academy of Sciences of The United States of America.
Fatty acid beta-oxidation in germinating Arabidopsis seeds is supported by peroxisomal hydroxypyruvate reductase when malate dehydrogenase is absent; Plant Molecular Biology
Functional multi-layer graphene-algae hybrid material formed using vortex fluidics; Green Chemistry
Genetic and chromosomal variation in Petunia hybrida plants regenerated from protoplast and callus cultures; Biologia Plantarum
Genetic Regulation of Shoot Architecture; Annual Review of Plant Biology
Glucan, water dikinase activity stimulates breakdown of starch granules by plastidial β-amylases; Plant Physiology
Glycoxylate cycle enzyme activities are induced in senescent pumpkin fruits; Plant Science
Glyoxylate cycle enzyme synthesis during the irreversible phase of senescence of cucumber cotyledons; Journal of Plant Physiology
Glyoxylate cycle enzymes in seedlings and in mature plants of tomato (Lycopersicon esculentum Mill.); Plant Science
Glyoxysomal malate synthase of cucumber: molecular cloning of a cDNA and regulation of enzyme synthesis during germination; Plant Physiology
Green hydrogen: algal biohydrogen production; Natural and Artificial Photosynthesis: Solar Power as an Energy Source
Hormone function in plants; Hormone Metabolism and Signaling in Plants
Hormone Metabolism and signaling in Plants; Academic Press, Elsevier
Identification of more than 200 glucose-responsive Arabidopsis genes none of which responds to 3-O-methylglucose or 6-deoxyglucose; Plant Molecular Biology
Identification of two Arabidopsis genes encoding a peroxisomal oxidoreductase-like protein and an acyl-CoA synthetase-like protein that are required for responses to pro-auxins; Plant Molecular Biology
Inhibition of Arabidopsis chloroplast β-amylase BAM3 by maltotriose suggests a mechanism for the control of transitory leaf starch mobilisation; PLoS One
Jasmonate signaling and remodeling of cell wall metabolism induced by boron deficiency in pea shoots; Environmental and Experimental Botany: An International Journal
KAI2- and MAX2-mediated responses to karrikins and strigolactones are largely independent of HY5 in Arabidopsis seedlings; Molecular Plant
Karrikin and cyanohydrin smoke signals provide clues to new endogenous plant signaling compounds; Molecular Plant
Karrikin-KAI2 signalling provides Arabidopsis seeds with tolerance to abiotic stress and inhibits germination under conditions unfavourable to seedling establishment; New Phytologist
Karrikins discovered in smoke trigger Arabidopsis seed germination by a mechanism requiring gibberellic acid synthesis and light; Plant Physiology
Karrikins enhance light responses during germination and seedling development in Arabidopsis thaliana; Proceedings of the National Academy of Sciences of The United States of America
Karrikins force a rethink of strigolactone mode of action; Plant Signaling and Behavior
Karrikins: a new family of plant growth regulators in smoke; Plant Science
Knockout of the two evolutionarily conserved peroxisomal 3-ketoacyl-CoA thiolases in Arabidopsis recapitulates the abnormal inflorescence meristem 1 phenotype; Journal of Experimental Botany
Leaf senescence is accompanied by an early disruption of the microtubule network in Arabidopsis; Plant Physiology
Leaves of the Arabidopsis maltose exporter1 mutant exhibit a metabolic profile with features of cold acclimation in the warm; PLoS ONE
Light-stimulated accumulation of transcripts of nuclear and chloroplast genes for ribulosebisphosphate carboxylase; Journal of Molecular and Applied Genetics
Lipid utilization, gluconeogenesis, and seedling growth in Arabidopsis mutants lacking the glyoxylate cycle enzyme malate synthase; Journal of Biological Chemistry
lnduction of malate synthase gene expression in senescent and detached organs of cucumber; Plant Cell
Malate Circulation: Linking Chloroplast Metabolism to Mitochondrial ROS; Trends in Plant Science
Metabolic regulation of glyoxylate-cycle enzyme synthesis in detached cucumber cotyledons and protoplasts; Planta
Methylation of ribosomal RNA genes in Petunia hybrida plants, callus cultures and regenerated shoots; Plant Cell Reports
Mitochondrial malate dehydrogenase lowers leaf respiration and alters photorespiration and plant growth in Arabidopsis; Plant Physiology
Molecular basis for high ligand sensitivity and selectivity of strigolactone receptors in Striga; Plant Physiology
Molecular cloning and sequencing of cDNA encoding the precursor to the small subunit of chloroplast ribulose-1,5-bisphosphate carboxylase; Nature
Molecular cloning of cucumber phosphoenolpyruvate carboxykinase and developmental regulation of gene expression; Plant Molecular Biology
Monosaccharide/proton symporter AtSTP1 plays a major role in uptake and response of Arabidopsis seeds and seedlings to sugars; The Plant Journal
Nine 3-ketoacyl-CoA thiolases (KATs) and acetoacetyl-CoA thiolases (ACATs) encoded by five genes in Arabidopsis thaliana are targeted either to peroxisomes or cytosol but not to mitochondria; Plant Molecular Biology
Nitrate removal from liquid effluents using microalgae immobilized on chitosan nanofiber mats; Green Chemistry
Nitrate uptake by p-phosphonic acid calix[8]arene stabilized graphene; Chemical Communications
Nitrate uptake by p-phosphonic acid or p-(trimethylammonium) methyl calix[8]arene stablized laminar materials; RSC Advances
Normal starch content and composition in tubers of antisense potato plants lacking D-enzyme (4-α-glucanotransferase); Planta
Novel proteins, putative membrane transporters, and an integrated metabolic network are revealed by quantitative proteomic analysis of Arabidopsis cell culture peroxisomes; Plant Physiology
Nuclear-plastid interactions in plastid protein synthesis; The fourth John Innes symposium, the plant genome, and second international haploid conference
Peroxisomal ATP-binding cassette transporter COMATOSE and the multifunctional protein ABNORMAL INFLORESCENCE MERISTEM are required for the production of benzoylated metabolites in Arabidopsis seeds; Plant Physiology
Peroxisomal hydroxypyruvate reductase is not essential for photorespiration in Arabidopsis but its absence causes an increase in the stoichiometry of photorespiratory CO2 release; Photosynthesis Research
Peroxisomal malate dehydrogenase is not essential for photorespiration in Arabidopsis but its absence causes an increase in the stoichiometry of photorespiratory CO2 release; Plant Physiology
Photoregulation of transcription of the nuclear and chloroplast genes for ribulose bisphosphate carboxylase; Society for Experimental Biology. Seminar Series
Plastidial α-glucan phosphorylase is not required for starch degradation in Arabidopsis leaves but has a role in the tolerance of abiotic stress; Plant Physiology
Postgerminative growth and lipid catabolism in oilseeds lacking the glyoxylate cycle; Proceedings of the National Academy of Sciences of The United States of America
Potato D-enzyme catalyzes the cyclization of amylose to produce cycloamylose, a novel cyclic glucan; Journal of Biological Chemistry
Primary products: Starch
Processing of small subunit precursor of ribulose bisphosphate carboxylase and its assembly into whole enzyme are stromal events; Nature
Production of the seed germination stimulant karrikinolide from combustion of simple carbohydrates; Journal of Agricultural and Food Chemistry
Q&A: What are strigolactones and why are they important to plants and soil microbes?; BMC Biology
Recombinant aequorin as a probe for cytosolic free Ca 2+ in Escherichia coli; FEBS Letters
Regulation of expression of the cucumber isocitrate lyase gene in cotyledons upon seed germination and by sucrose; Plant Molecular Biology
Regulation of seed germination and seedling growth by chemical signals from burning vegetation; Annual Review of Plant Biology
Regulation of the cholesterol biosynthetic pathway and its integration with fatty acid biosynthesis in the oleaginous microalga Nannochloropsis oceanica; Biotechnology for Biofuels
Reporter gene-facilitated detection of compounds in Arabidopsis leaf extracts that activate the karrikin signaling pathway; Frontiers in Plant Science
Requirement for 3-ketoacyl-CoA thiolase-2 in peroxisome development, fatty acid; The Plant Journal
Requirement for the plastidial oxidative pentose phosphate pathway for nitrate assimilation in Arabidopsis; The Plant Journal
Rice cytochrome P450 MAX1 homologs catalyze distinct steps in strigolactone biosynthesis; Nature Chemical Biology
Rice Plant Architecture: Molecular Basis and Application in Breeding; Rice Genomics, Genetics and Breeding
Role of malate synthase and the glyoxylate cycle in oilseed plants; Plant Molecular Biology Reporter
Roles of cell-wall invertases and monosaccharide transporters in the growth and development of Arabidopsis; Journal of Experimental Botany
Seasonal control of seed germination; New Phytologist
Signaling from the endoplasmic reticulum activates brassinosteroid signaling and promotes acclimation to stress in Arabidopsis; Science Signaling
Signalling and responses to strigolactones and karrikins; Current Opinion in Plant Biology
Similar protein phosphatases control starch metabolism in plant and glycogen metabolism in mammals; Journal of Biological Chemistry
SLR1 inhibits MOC1 degradation to coordinate tiller number and plant height in rice; Nature Communications
Smoke signals and seed dormancy: where next for MAX2?; Plant Signaling and Behavior
Solar irradiation of the seed germination stimulant karrikinolide produces two novel head-to-head cage dimers; Organic & Biomolecular Chemistry
Specialisation within the DWARF14 protein family confers distinct responses to karrikins and strigolactones in Arabidopsis; Development
Species-specific repeated DNA sequences from Petunia; Plant Science
Starch degradation; Annual Review of Plant Biology
Starch degradation in leaves; Journal of Applied Glycoscience
Starch mobilization in leaves; Journal of Experimental Botany
Starch: not so sticky; Biological Sciences Review
Stereospecificity in strigolactone biosynthesis and perception; Planta
Strain-induced 'band flips' in cyclodecaamylose and higher homologues; Angewandte Chemie International Edition
Strigolactone and Karrikin Signaling Pathways Elicit Ubiquitination and Proteolysis of SMXL2 to Regulate Hypocotyl Elongation in Arabidopsis; The Plant Cell
Strigolactone hormones and their stereoisomers signal through two related receptor proteins to induce different physiological responses in Arabidopsis; Plant Physiology
Strigolactone signaling in Arabidopsis regulates shoot development by targeting D53-like SMXL repressor proteins for ubiquitination and degradation; Plant Cell
Strigolactones redefine plant hormones; Science China: Life Sciences
Strigolactones: destruction-dependent perception?; Current Biology
Structure-activity relationship of karrikin germination stimulants; Journal of Agricultural and Food Chemistry
Structures of the common cyclodextrins and their larger analogues - beyond the doughnut; Chemical Reviews
Substrate-induced degradation of the a/b-fold hydrolase KARRIKIN INSENSITIVE2 requires a functional catalytic triad but is independent of MAX2; Molecular Plant
Sugar effect on the malate synthase and isocitrate lyase gene expression at the level of mRNA stability; Asia-Pacific Journal of Molecular Biology and Biotechnology
SUPPRESSOR OF MORE AXILLARY GROWTH2 1 controls seed germination and seedling development in Arabidopsis; Plant Physiology
Synthesis and Function of Glyoxylate Cycle Enzymes; Plant Peroxisomes
Synthesis of the small subunit of ribulose-bisphosphate carboxylase from genes cloned into plasmids containing the SP6 promoter; Biochemical Journal
Synthesis, Transport and Assembly of Chloroplast Proteins; Genome Organization and Expression in Plants
The Arabidopsis 3-ketoacyl-CoA thiolase-2 (kat2-1) mutant exhibits increased flowering but reduced reproductive success; Journal of Experimental Botany
The Arabidopsis ortholog of rice DWARF27 acts upstream of MAX1 in the control of plant development by strigolactones; Plant Physiology
The breakdown of starch in leaves; New Phytologist
The crystal structure of a plant 3-ketoacyl-CoA thiolase reveals the potential for redox control of peroxisomal fatty acid β-oxidation; Journal of Molecular Biology
The diurnal metabolism of leaf starch; Biochemical Journal
The function of 4-α-glucanotransferases and their use for the production of cyclic glucans; Biotechnology and Genetic Engineering Reviews
The genetic dissection of a short-term response to low CO2 supports the possibility for peroxide-mediated decarboxylation of photorespiratory intermediates in the peroxisome; Molecular Plant
The heteromultimeric debranching enzyme Involved in starch synthesis in Arabidopsis requires both isoamylase1 and isoamylase2 subunits for complex stability and activity; PLoS ONE
The karrikin response system of Arabidopsis; The Plant Journal
The major chlorophyll a/b binding protein of petunia is composed of several polypeptides encoded by a number of distinct nuclear genes; Journal of Molecular and Applied Genetics
The malate synthase gene of cucumber; Plant Molecular Biology
The metabolome of Chlamydomonas reinhardtii following induction of anaerobic H2 production by sulfur depletion; Journal of Biological Chemistry
The non-essential role of peroxisomal malate dehydrogenase during photorespiration; Photosynthesis Research
The nucleotidase/phosphatase SAL1 is a negative regulator of drought tolerance in Arabidopsis; The Plant Journal
The origins and mechanisms of karrikin signalling; Current Opinion in Plant Biology
The priming of amylose synthesis in Arabidopsis leaves; Plant Physiology
The role of D-enzyme in starch metabolism in plants; Journal of Applied Glycoscience
The structure of the karrikin-insensitive protein (KAI2) in Arabidopsis thaliana; PLoS One
The synthesis and biological evaluation of labelled karrikinolides for the elucidation of the mode of action of the seed germination stimulant; Tetrahedron
The Synthesis and Degradation of Starch in Arabidopsis Leaves: The Role of Disproportionating Enzyme; Starch: Advances in Structure and Function
Transformation of chilli pepper (Capsicum frutescens) with a phenylalanine ammonia-lyase gene; Plant Biotechnology
Transgenic plant aequorin reports the effects of touch and cold-shock and elicitors on cytoplasmic calcium; Nature
Using gene knockouts to investigate plant metabolism; Journal of Experimental Botany
V-Amylose at atomic resolution: X-ray structure of a cycloamylose with 26 glucose residues (cyclomaltohexaicosaose); Proceedings of the National Academy of Sciences of The United States of America
Variation in organisation and copy number of ribosomal RNA genes in Petunia hybrida somaclones; Biologia Plantarum
Vortex fluidic entrapment of functional microalgal cells in a magnetic polymer matrix; Nanoscale
What are karrikins and how were they ‘discovered’ by plants?; BMC Biology
When is a peroxisome not a peroxisome?; Trends in Plant Science
Wind-induced plant motion immediately increases cytosolic calcium; National Academy of Sciences of The United States of America. Proceedings
Witchcraft and destruction; Nature

Research Projects

Advanced high resolution biomolecular analysis facility for Tasmania; Australian Research Council (ARC)
ARC Centre of Excellence for Plant Success in Nature and Agriculture; Australian Research Council (ARC)
Clocks in crops: exploring the role of circadian rhythms in crop adaptation; Australian Research Council (ARC)
Marine Bioproducts Cooperative Research Centre; Department of Industry, Innovation and Science (DoIIS)
Understanding the biological functions of the karrikin-responsive signaling system of plants in growth, development and responses to the environment; Australian Research Council (ARC)
Engagement and Impact appointment for Prof Steven Smith

Research Candidate Supervision