Web Access Research Portal

Researcher: Crosbie, PBB (Dr Philip Crosbie)

Research Fields

Aquaculture
Fish Pests and Diseases
Veterinary Anaesthesiology and Intensive Care
Phylogeny and Comparative Analysis
Infectious Agents
Microbial Ecology
Microbiology
Veterinary Microbiology (excl. Virology)
Veterinary Parasitology
Veterinary Immunology
Veterinary Epidemiology

Research Impact

Fisheries - Aquaculture
Aquaculture Fin Fish (excl. Tuna)
Aquaculture Tuna
Aquaculture Crustaceans (excl. Rock Lobster and Prawns)
Fisheries - Wild Caught
Marine Flora, Fauna and Biodiversity

Career Best Publications

Neoparamoeba branchiphila n. sp., and related species of the genus Neoparamoeba Page, 1987: morphological and molecular characterization of selected strains; Journal of Fish Diseases
Immune responses of barramundi, Lates calcarifer (Bloch), after administration of an experimental Vibrio harveyi bacterin by intraperitoneal injection, anal intubation and immersion; Journal of Fish Diseases
Production of polyclonal antisera against barramundi (Lates calcarifer Bloch) serum immunoglobulin derived from affinity columns containing mannan-binding protein or staphylococcal protein A; Aquaculture
The immuno-adjuvant effect of carp interleukin-1 beta on the humoral immune response of barramundi, Lates calcarifer (Bloch); Journal of Fish Diseases
The induction of laboratory-based amoebic gill disease revisited; Journal of Fish Diseases

Research Publications

Neoparamoeba branchiphila n. sp., and related species of the genus Neoparamoeba Page, 1987: morphological and molecular characterization of selected strains; Journal of Fish Diseases
Neoparamoeba perurans loses virulence during clonal culture; International Journal for Parasitology
Neoparamoeba perurans n. sp., an agent of amoebic gill disease of Atlantic Salmon (Salmo salar); International Journal for Parasitology
A culture-dependent 16S rRNA gene-based approach to identify gill bacteria associated with amoebic gill disease in Atlantic salmon; European Association of Fish Pathologists. Bulletin
A preliminary study into effects of seawater acclimation on the progression of Amoebic Gill Disease (AGD) in out of season Atlantic salmon (Salmo salar, L.); European Association of Fish Pathologists. Bulletin
Administration of recombinant attachment protein (r22C03) of Neoparamoeba perurans induces humoral immune response against the parasite in Atlantic salmon (Salmo salar); Fish and Shellfish Immunology
AGD vaccine: an antibody approach; Aquafin CRC Conference
Amoebic gill disease (AGD) of Atlantic salmon (Salmo salar) in Tasmania: An Epidemiological Challenge; ISVEE XI Innovation: Reshaping Veterinary Epidemiology
Amoebic gill disease in cultured salmonids in Australia; 4th International Symposium on Aquatic Animal Health
Amoebic gill disease in hatchery-reared ayu, Plecoglossus altivelis(Temminck & Schlegel), in Japan is caused by Neoparamoeba perurans; Journal of Fish Diseases
Amoebic Gill Disease research highlights; The Second scientific conference of the Atlantic salmon aquaculture subprogram
Amoebic gill disease resistance is not related to the systemic antibody response of Atlantic salmon, Salmo salar L; Journal of Fish Diseases
An introduction to amoebic gill disease of Atlantic salmon (Salmo salar L.); 2nd FRDC Aquatic Animal Health Subprogram Scientific Conference
An optimized real-time PCR for the detection and quantification of viable Neoparamoeba perurans using propidium monoazide; Seventh International Symposium on Aquatic Animal Health
Atlantic salmon (Salmo salar L.) exposed to cultured gill-derived Neoparamoeba branchilila fail to develop amoebic gill disease (AGD); European Association of Fish Pathologists. Bulletin
Changes in antigenic profile during culture of Neoparamoeba sp., causative agent of amoebic gill disease in Atlantic salmon; International Journal for Parasitology
Characterisatiion of an immunodominant, high molecular weight glycoprotein on the surface of infectious Neoparamoeba ssp., causative agent of amoebic gill disease (AGD) in Atlantic salmon; Fish and Shellfish Immunology
Characterisation of host - and environment -isolated strains of Neoparamoeba pemaquidensis, the causative organism of amoebic gill disease; Diseases of Fish and Shellfish
Concentration effects of Winogradskyella sp. on the incidence and severity of amoebic gill disease; Diseases of Aquatic Organisms
Concurrent epizootic hyperinfections of sea lice (predominantly Caligus chiastos) and blood flukes (Cardicola forsteri) in ranched Southern Bluefin tuna; Veterinary Parasitology
Cultured gill-derived Neoparamoeba pemaquidensis fails to elicit amoebic gill disease (AGD) in Atlantic salmon Salmo salar; Diseases of Aquatic Organisms
Detection of Neoparamoeba pemaquidensis in marine sediments in Tasmania; The Second scientific conference of the Atlantic salmon aquaculture subprogram
Detection of Neioparamoeba pemaquidensis in marine sediments in Tasmania; The Annual Scientific Meeting of the Australian Society for Parasitiology Inc.
Detection of Neoparamoeba pemaquidensis in marine sediments in Tasmania; Aquafin CRC Conference
Development of an in vivo assay to assess attachment of Neoparamoeba sp. (an amphizoic gymnamoeba) to the gills of Atlantic salmon, Salmo salar L; Journal of Fish Diseases
Differentially expressed proteins in gill and skin mucus of Atlantic salmon (Salmo salar) affected by amoebic gill disease; Fish and Shellfish Immunology
Distribution of Neoparamoeba sp. in sediments around marine finfish farming sites in Tasmania; Diseases of Aquatic Organisms
Effect of immunization route on mucosal and systemic immune response in Atlantic salmon (Salmo salar); Veterinary Immunology and Immunopathology: An International Journal of Comparative Immunology
Effects of anaesthetics containing eugenol on Neoparamoeba perurans; Aquaculture
Effects of different batches of Neoparamoeba perurans and fish stocking densities on the severity of amoebic gill disease in experimental infection of Atlantic salmon, Salmo salar L; Aquaculture Research
Effects of epitheliocystis on serum lysozyme activity and osmoregulation in cultured juvenile striped trumpeter, Latris lineata (Forster); Aquaculture
Effects of single and repeated infections with Neoparamoeba perurans on antibody levels and immune gene expression in Atlantic salmon (Salmo salar); Fish and Shellfish Immunology
Environmental factors and chemical agents affecting the growth of the pathogenic marine ciliate Uronema nigricans; Diseases of Aquatic Organisms
Evidence of immune and inflammatory processes in the gills of AGD-affected Atlantic salmon, Salmo salar L; Fish and Shellfish Immunology
Evidence of immune and inflammatory processes in the gills of AGD-affected Atlantic salmon, Salmo salar L. ; Seventh International Symposium on Aquatic Animal Health
Final Report Aquafin CRC Comercial in Confidence SOUTHERN BLUEFIN TUNA AQUACULTURE SUBPROGRAM: IMPROVING HUSBANDRY AND PERFORMANCE OF SOUTHERN BLUEFIN TUNA THROUGH BETTER UNDERSTANDIING OF THE RELATIONSHIP BETWEEN FISH STRESS AND HEALTH
First record of amoebic gill disease caused by Neoparamoeba perurans in South Africa; Journal of Fish Diseases
FRDC ASBTIA: Investigation of causes of mortalities in ranched SBT (southern blluefin tuna, Thunnus maccoyii)
Host-Pathogen interactions in amoebic gill disease
Hydrogen peroxide treatment of experimentally induced amoebic gill disease; 1st FRDC Australasian Animal Health Conference
Immune response of Barramundi to vibro harveyi bacterin
Immune response of barramundi(Lates calcarifer) to vibrio harveyi bacterin
Immune responses in salmonids affected by amoebic gill disease; Fifth International Symposium on Aquatic Animal Health
Immune responses of barramundi, Lates calcarifer (Bloch), after administration of an experimental Vibrio harveyi bacterin by intraperitoneal injection, anal intubation and immersion; Journal of Fish Diseases
Immunity to Amoeba; Developmental and Comparative Immunology
Important breakthrough in Salmon DiseaseResearch
In vitro cultured Neoparamoeba perurans causes amoebic gill disease in Atlantic salmon and fulfils Koch's postulates; International Journal for Parasitology
Insights into Amoebic Gill Disease pathogenesis
Isolation of Neoparamoeba pemaquidensis Page, 1987 from Marine and Estuarine Sediments in Tasmania; European Association of Fish Pathologists
Model Development for Epidemiology of Amoebic Gill disease
Oral vaccination of first-feeding Atlantic salmon, Salmo salar L., confers greater protection against yersiniosis than immersion vaccination; Vaccine
Phylogeny of Neoparamoeba strains isolated from marine fish and invertebrates as inferred from SSU rDNA sequences; Diseases of Aquatic Organisms
Preliminary success using hydrogen peroxide to treat Atlantic salmon Salmo salar L. affected with experimentally induced amoebic gill disease (AGD); Journal of Fish Diseases
Production of polyclonal antisera against barramundi (Lates calcarifer Bloch) serum immunoglobulin derived from affinity columns containing mannan-binding protein or staphylococcal protein A; Aquaculture
Rapid detection and quantification of Neoparamoeba perurans in the marine environment; Aquaculture
Serological evidence of an antibody response in farmed southern bluefin tuna naturally infected with the blood fluke Cardicola forsteri; Fish and Shellfish Immunology
Support for the coevolution of Neoparamoeba and their endosymbionts, Perkinsela amoebae-like organisms; European Journal of Protistology
The immuno-adjuvant effect of carp interleukin-1 beta on the humoral immune response of barramundi, Lates calcarifer (Bloch); Journal of Fish Diseases
The induction of laboratory-based amoebic gill disease revisited; Journal of Fish Diseases
Use of flow cytometry to screen chemotherapeutants with portential to control of Neoparamoeba pemaquidensis infection in Atlantic salmon, Salmo salar; Australasian flow Cytometry Group 25th Annual Scientific Meeting
Use of potassium permanganate to disinfect Atlantic salmon (Salmo salar) gills; Bulletin European Association of Fish Pathologists
Vaccination with recombinant protein (r22C03), a putative attachment factor of Neoparamoeba perurans, against AGD in Atlantic salmon (Salmo salar) and implications of a co-infection with Yersinia ruckeri; Fish and Shellfish Immunology

Research Projects

Aquafin CRC - AGD Vaccine Phase III-Sea-based Trials, Vaccine Refinement and Commercialisation; Fisheries Research & Development Corporation (FRDC)
Aquafin CRC - Atlantic Salmon Aquaculture Subprogram: Establishment of Challenge for AGD; Fisheries Research & Development Corporation (FRDC)
Atlantic Salmon Aquaculture Subprogram: Extension Funding Application- AGD Vaccine Phase III; Fisheries Research & Development Corporation (FRDC)
Atlantic Salmon Subprogram: Hydrogen peroxide treatment of Atlantic salmon affected by AGD; Fisheries Research & Development Corporation (FRDC)
For the Atlantic Salmon Subprogram - Culture and cryopreservation of Neoparamoeba perurans (AGD); Fisheries Research & Development Corporation (FRDC)
Using the Mucosal Antibody Response to Recombinant Neoparamoeba perurans Attachment Proteins to Design an Experimental Vaccine for Amoebic Gill Disease; Australian Seafood Cooperative Research Centre (ASCRC)
Literature Review - chinook salmon
Supply of amoebae attached to Petri dishes for in vitro trials at Huon Aquaculture to be carried out by Ridley AgriProducts Pty Ltd
Tenacibaculum maritimum vaccine trial

Research Candidate Supervision

Assessment of Resistance to Amoebic Gill Disease in the Tasmanian Atlantic Salmon Selective Breeding Population
Effect of Vaccination on Fish Immune Response
Environmental Reservoirs of Atlantic Salmon Pathogens Durine Marine Grow-Out
Investigation of New Approaches to Management of Salmonid Disease Through the Development and Use of Probiotics
Melanomacrophage centres in Australian fish
Role of Bacteria in Amoebic Gill Disease
Understanding Host-Pathogen in Salmo Salar
Using the Mucosal Response to Recombinant Neoparamoeba perurans Attachment Proteins to Design an Experimental Vaccine against Amoebic Gill Disease (AGD)